Bioinformatics and Stats
Just get your amplicon data back from the sequencer and wondering what to do next? Here’s our workflow
Wondering how to cull your amplicon data in a standardized way for quality control? Here’s our workflow.
Here’s a Rmarkdown with some commonly used community ecology statistics for amplicon data.
Molecular Protocols
2X CTAB Buffer
Diluting primers
DNA extraction from orchid roots
DNA extraction with Extract-N-Amp
PCR amplification from orchid roots
PCR amplification with ITS 1F & 4
PCR with AMF primers AML2/WANDA + nextera adapters
ExoSap PCR Clean up
Bead PCR Clean up
Automated Bead PCR Clean up for Nextera workflow
Gel electrophoresis
Sanger Sequencing with GeneWiz or ASGPB
Weighing
Soil weighing for DNA extraction
Root weighing for DNA extraction
Assays
Glycerol cryopreservation of yeast fungi
Hot staining of AMF roots
Quantum dot conjugation
Arbuscular Mycorrhizal Spore Extractions
Root mounting for microscopy
Vital staining of spores
Soil sampling for trap cultures (movie)
Setting up trap cultures (movie)
Peloton extraction, culturing, isotope analysis, DNA isolation from orchid roots
Peloton extraction and culturing #2
Symbiotic orchid germination with mycorrhizal fungi
Biosafety Protocols
Field packing list
Safe handling of soil
Rapid Ohia Death precautions
Here are resources for colleagues, postdocs, graduate students, and others in the field of fungal ecology.
Commonly used protocols in the Hynson Lab
Grant and fellowship opportunities to fund Graduate and Postdoctoral studies.
Tips on how to succeed in graduate school, effective teaching, communicating science, getting a job, and other professional development resources.
Links to fungal labs, isotope labs, bioinformatics and databases, and societies, clubs, and networks.
Courses currently taught by Dr. Hynson and some details on our lab’s outreach activities.
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